Biotechnology techniques Assignment Example | Topics and Well Written Essays - 1500 words

Biotechnology techniques - Assignment ExampleIs this statement TRUE or preposterous, explain? (5 points) Electroporation is employ to introduce DNA in prokaryotic and eukaryotic cells. This is the general method of introducing the exotic DNA into the coveted cells. This method uses the electrical pulses to create a pore in the cell membrane and through these pores the foreign DNA molecule is inserted. 3. What argon the 3 main sources of paper Cells? (10 points) The 3 main sources of stem cells include machinate bone marrow, Bloodstream or peripheral blood and finally umbilical cord blood from the newborns. Bone marrow particularly those in the pelvis bones have rice supply. Blood normally has fewer stem cells, and when hormone like substances called growth factors are given to individuals, stems cells grow faster and move from the marrow to the blood. Blood left in the placenta and the umbilical cord of the newborns cigarette be retrieved, store and utilized for future. 4. W hat is meant by humanization of mouse antibodies and what is the need for humanization?(10 points) While using therapeutic antibodies or murine antibodies disjointed from mice in humans, immunogencity problem arose due to the differing protein sequence. To alleviate this issue, specific regions within a mouse antibody called complementarity determining regions (CDR) are identified and transferred into human variable regions and this process is called humanization. When this is done, it will confer binding and so the resultant humanized antibodies can function without major immunogencity problem. 5. Which technology cannot be apply to determine the function of a gene? Explain. (5 points) Stem cell technology cannot be used to study the gene function because, it is a method to turn the entire cell and study the organs and it is not the direct method of analysis of gene function. 6. complementary DNA microarray technology specifically evaluates the gene expression levels of ONLY m icroRNA in cells and tissues. Is this statement TRUE or FALSE? Explain? (5 points) No cDNA microarray technology is used for the analysis of all the genes and its function. cDNA technology is used to analyse the functions of known and unknown gene. 7. Which of the following gene expression strategies increase the number of diametrical proteins lendable to the cell without increasing the total number of genes encoded by the genome?(5 points) Translation is used to increase the number of different proteins in the cell without increasing the total number of genes in the genome. By varying the mRNA translation, the protein implication is modified. 8. In the sequence given below, how many EcoRI recognition sites are present? Please circle them. (10 points) 5-ATGGAATTCCCAATTGAATTCCGGCTGAATTCAAA-3 3-TACCTTAAGGGTTAACTTAAGGCCGACTTAAGTTT-5 There are three EcoRI recognition sites in the given sequences. They are 5-ATGGAATTCCCAATTGAATTCCGGCTGAATTCAAA-3 3-TACCTTAAGGGTTAACTTAAGGCCGACTTAAGTTT -5 9. What is meant by the statement, Complementation screening of genomic eukaryotic clones will not work in E.coli because of absence of lap joint machinery? (10 points) E.coli is a prokaryote. The bacterial systems cannot remove the introns from the mRNA sequences. Complementation screening is the technique used to identify the mutations in the gene of interest. In this method, the selection is based on the capacity of the gene to get back the phenotype. The splice mechanism is not present in the